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Improving data quality and preserving HCD-generated reporter ions with EThcD for isobaric tag-based quantitative proteomics and proteome-wide PTM studies

1 month ago   |   By Harvard

Q. Yu, X. Shi, Y. Feng, K. C. Kent, and L. Li. 2017. “ Improving data quality and preserving HCD-generated reporter ions with EThcD for isobaric tag-based quantitative proteomics and proteome-wide PTM studies .” Anal Chim Acta, 968, Pp. 40-49. Abstract Mass spectrometry -based isobaric labeling has undergone rapid development in recent years due to its capability for high throughput quantitation. Apart from its originally designed use with collision-induced dissociation and higher-energy collisional dissociation , isobaric tagging technique could also work with electron-transfer...
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Evaluation and Application of Dimethylated Amino Acids as Isobaric Tags for Quantitative Proteomics of the TGF-beta/Smad3 Signaling Pathway

1 month ago   |   By Harvard

Q. Yu, X. Shi, T. Greer, C. B. Lietz, K. C. Kent, and L. Li. 2016. “ Evaluation and Application of Dimethylated Amino Acids as Isobaric Tags for Quantitative Proteomics of the TGF-beta/Smad3 Signaling Pathway .” J Proteome Res, 15, Pp. 3420-31. Abstract Isobaric labeling has become a widespread tool for quantitative proteomic studies. Here, we report the development and evaluation of several dimethylated amino acids as novel isobaric tags for quantitative proteomics. Four-plex dimethylated alanine , valine , and leucine have been synthesized, sharing common features of peptide tagging and...
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